Conformational flexibility of metazoan fatty acid synthase enables catalysis
نویسندگان
چکیده
منابع مشابه
Arabidopsis thaliana dehydroascorbate reductase 2: Conformational flexibility during catalysis
This corrects the article DOI: 10.1038/srep42494.
متن کاملThyroid hormone responsive protein Spot14 enhances catalysis of fatty acid synthase in lactating mammary epithelium.
Thyroid hormone responsive protein Spot 14 has been consistently associated with de novo fatty acid synthesis activity in multiple tissues, including the lactating mammary gland, which synthesizes large quantities of medium chain fatty acids (MCFAs) exclusively via FASN. However, the molecular function of Spot14 remains undefined during lactation. Spot14-null mice produce milk deficient in tota...
متن کاملExperimental verification of conformational variation of human fatty acid synthase as predicted by normal mode analysis.
Fatty acid synthase (FAS) is a 550 kDa homodimeric enzyme with multiple functional and structural domains. Normal mode analysis of a previously determined 19 A structure of FAS suggested that this enzyme might assume different conformational states with several distinct hinge movements. We have used a simultaneous multiple-model refinement method to search for the presence of the structural con...
متن کاملFluorescence studies of chicken liver fatty acid synthase. Segmental flexibility and distance measurements.
The 4'-phosphopantetheine of chicken liver fatty acid synthase was specifically labeled with the fluorescent substrate analog coenzyme A 6-[7-nitrobenz-2-oxa-1,3-diazol-4-yl]aminohexanoate at low salt concentrations. A serine at the active site of the thioesterase was specifically labeled with the fluorescent compounds 6-[7-nitrobenz-2-oxa-1,3-diazol-4-yl]aminopentylmethylphosphono fluoridate a...
متن کاملMolecular Pathways: Fatty Acid Synthase.
Therapies that target tumor metabolism represent a new horizon in anticancer therapies. In particular, cancer cells are dependent on the generation of lipids, which are essential for cell membrane synthesis, modification of proteins, and localization of many oncogenic signal transduction enzymes. Because fatty acids are the building blocks of these important lipids, fatty acid synthase (FASN) e...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Structural & Molecular Biology
سال: 2009
ISSN: 1545-9993,1545-9985
DOI: 10.1038/nsmb.1532